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MJ Research ptc-100 programmable thermal controller
Ptc 100 Programmable Thermal Controller, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/programmable+thermal+controller/ptc+100+thermocycler/pmc11884422-46-20-24
Average 90 stars, based on 1 article reviews
ptc-100 programmable thermal controller - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing
Article Snippet: The extracted RNA was used to prepare cDNA using a cDNA synthesis kit (Applied Biosystems, Foster City, CA, United States). .. The cDNA reverse-transcription reaction was performed using a programmable thermal controller (MJ Research, St. Bruno, PQ, Canada) programmed to one cycle at 42°C for 30 min, followed by activation at 95°C for 2 min, after which the cDNA was stored at −20°C for future use. ..

Article Title: Inhibition of AcrAB-TolC enhances antimicrobial activity of phytochemicals in Pectobacterium brasiliense
Article Snippet: The extracted RNA was used to prepare cDNA using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Thermo Fisher Scientific Inc, Carlsbad, CA, USA). .. The cDNA reverse-transcription reaction was performed using a programmable thermal controller (MJ Research, St. Bruno, PQ, Canada) programmed to one cycle as step1: 25°C for 10 min, step 2: 37°C for 120 min, step 3: 85°C for 5 min and step 4: 10°C after which the cDNA was stored at -20°C for future use. ..

Article Title: Long non-coding RNA generated from CDKN1A gene by alternative polyadenylation regulates p21 expression during DNA damage response.
Article Snippet: Total RNA was extracted using TRIzol ( Invitrogen ) according to the manufacturer’s instructions as described ( 34 ) from all the cells used in this study that were mock- or treated ( UV, Actinomycin D, etoposide, cycloheximide ) . .. Reverse transcription ( RT ) was performed with M-MLV reverse transcriptase ( Promega ) in a programmable thermal controller ( MJ Research ) . .. RNA was quantified using nanodrop and 100 ng of A 260 / A 280 > 1.95 of freshly prepared or one-time thawed RNA was used for reverse transcription.

Activation Assay:

Article Title: Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing
Article Snippet: The extracted RNA was used to prepare cDNA using a cDNA synthesis kit (Applied Biosystems, Foster City, CA, United States). .. The cDNA reverse-transcription reaction was performed using a programmable thermal controller (MJ Research, St. Bruno, PQ, Canada) programmed to one cycle at 42°C for 30 min, followed by activation at 95°C for 2 min, after which the cDNA was stored at −20°C for future use. ..

Incubation:

Article Title: Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks
Article Snippet: The total RNA concentration was measured using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific). .. The samples were incubated with RQ1 RNase-free DNase (Promega Co., Madison, WI, USA) on a programmable thermal controller (PTC-100; MJ Research, Waltham, MA, USA) according to the manufacturer’s instructions. .. Total RNA samples were reverse-transcribed using Rever-Tra Ace (Toyobo Co., Ltd., Osaka, Japan) according to the manufacturer’s instructions.

Polymerase Chain Reaction:

Article Title: Ras Inhibition Induces Insulin Sensitivity and Glucose Uptake
Article Snippet: .. PCR was carried out with ReddyMixTM PCR Master Mix (ABgene) on a Programmable Thermal Controller (MJ Research, Waltham, MA) at gene-specific conditions. ..

Article Title: TIP41L, a putative candidate gene conferring both seed size and boll weight, was fine-mapped in an introgression line of Gossypium hirsutum-Gossypium arboreum.
Article Snippet: QTLs for yield-related traits in tetraploid cotton have been widely mapped, but QTLs introduced from diploid species into tetraploid cotton background remain uninvolved.. Here, a stable introgression line with the traits of small boll and seed on Chr.. A12, IL197 derived from Gossypium hirsutum (2n = AADD = 52) × Gossypium arboreum (2n = AA = 26), was employed to construct the F2 and F3 secondary populations for fine-mapping QTLs of yield-related traits.

Article Title: Evaluation of the antifungal activity of essential oils against <i>Alternaria alternata</i> causing fruit rot of <i>Eriobotrya japonica</i>
Article Snippet: .. DNA amplification was performed by PCR using the primers specific for ITS for molecular identification of Alternaria sp. The polymerase chain reaction was carried out in MJ Research PTC-100TM Programmable Thermal Controller. ..

Electrophoresis:

Article Title: TIP41L, a putative candidate gene conferring both seed size and boll weight, was fine-mapped in an introgression line of Gossypium hirsutum-Gossypium arboreum.
Article Snippet: QTLs for yield-related traits in tetraploid cotton have been widely mapped, but QTLs introduced from diploid species into tetraploid cotton background remain uninvolved.. Here, a stable introgression line with the traits of small boll and seed on Chr.. A12, IL197 derived from Gossypium hirsutum (2n = AADD = 52) × Gossypium arboreum (2n = AA = 26), was employed to construct the F2 and F3 secondary populations for fine-mapping QTLs of yield-related traits.

Silver Staining:

Article Title: TIP41L, a putative candidate gene conferring both seed size and boll weight, was fine-mapped in an introgression line of Gossypium hirsutum-Gossypium arboreum.
Article Snippet: QTLs for yield-related traits in tetraploid cotton have been widely mapped, but QTLs introduced from diploid species into tetraploid cotton background remain uninvolved.. Here, a stable introgression line with the traits of small boll and seed on Chr.. A12, IL197 derived from Gossypium hirsutum (2n = AADD = 52) × Gossypium arboreum (2n = AA = 26), was employed to construct the F2 and F3 secondary populations for fine-mapping QTLs of yield-related traits.

DNA Amplification:

Article Title: Evaluation of the antifungal activity of essential oils against <i>Alternaria alternata</i> causing fruit rot of <i>Eriobotrya japonica</i>
Article Snippet: .. DNA amplification was performed by PCR using the primers specific for ITS for molecular identification of Alternaria sp. The polymerase chain reaction was carried out in MJ Research PTC-100TM Programmable Thermal Controller. ..



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